Hotspot

trnE-UUC-trnT-GGU

LSC · 32129-32629 · 501 bp

Back to Aponogeton

Pi 0.0293

MarkerSeek score 68.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 21
pi: 0.0293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	588-591 bp	1.0	82.6
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	586-589 bp	1.0	82.6
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	567-570 bp	1.0	82.3
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	565-568 bp	1.0	82.3
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	642-645 bp	1.0	82.2