Hotspot

psbI-trnS-GCU

LSC · 8774-8888 · 115 bp

Back to Aponogeton

Pi 0.0464

MarkerSeek score 71.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 8
pi: 0.0464
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGACCCAGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	228 bp	1.0	82.0
psbI-trnS-GCU_p2	TATCTAATGACCCAGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	229 bp	1.0	82.0
psbI-trnS-GCU_p3	ATCTAATGACCCAGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	229 bp	1.0	82.0
psbI-trnS-GCU_p4	TATCTAATGACCCAGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	230 bp	1.0	82.0
psbI-trnS-GCU_p5	TCTAATGACCCAGGGCGTAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	227 bp	1.0	81.7