Hotspot

rps16-trnQ-UUG

LSC · 6350-7536 · 1187 bp

Back to Aponogeton

Pi 0.0319
MarkerSeek score 70.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1187
variable_sites
56
pi
0.0319
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TGGTCCTGTTATTCGGAGGT 45.0 50.0 58.1 58.1 1255-1379 bp 1.0 83.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1240-1364 bp 1.0 83.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1244-1368 bp 1.0 79.0
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TGGTCCTGTTATTCGGAGGT 50.0 50.0 57.2 58.1 1247-1371 bp 1.0 78.7
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1232-1356 bp 1.0 78.6