Hotspot

trnH-GUG-psbA

LSC · 79-459 · 381 bp

Back to Aponogeton

Pi 0.0595

MarkerSeek score 88.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 32
pi: 0.0595
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	427-514 bp	1.0	88.0
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	430-517 bp	1.0	87.9
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	482-569 bp	1.0	86.5
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	484-571 bp	1.0	86.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AGCTGCTGTTGAAGTTCCAT	55.0	45.0	58.1	57.7	416-503 bp	1.0	86.4