Hotspot

trnN-GUU-trnR-ACG

IRa · 124876-125405 · 530 bp

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Pi 0.0151

MarkerSeek score 65.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 530
variable_sites: 8
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	671-707 bp	1.0	79.3
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	622-658 bp	1.0	78.1
trnN-GUU-trnR-ACG_p3	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	626-662 bp	1.0	77.3
trnN-GUU-trnR-ACG_p4	TCTCCCGTTGTTCCTCAGTA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	675-711 bp	1.0	76.9
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	665-701 bp	1.0	76.8