Hotspot

ndhF-rpl32

SSC · 108347-108991 · 645 bp

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Pi 0.00939

MarkerSeek score 65.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 645
variable_sites: 6
pi: 0.00939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACGCTGTCCAATATCCCTTC	50.0	50.0	58.0	57.4	828-935 bp	1.0	75.9
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	ACGCTGTCCAATATCCCTTC	45.0	50.0	58.0	57.4	826-933 bp	1.0	75.8
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	ACGCTGTCCAATATCCCTTC	45.0	50.0	58.0	57.4	827-934 bp	1.0	75.8
ndhF-rpl32_p4	GTTTTGTTGCCGTCGGAAAA	ACGCTGTCCAATATCCCTTC	45.0	50.0	58.1	57.4	833-940 bp	1.0	75.4
ndhF-rpl32_p5	AAACTGTTTTGTTGCCGTCG	ACGCTGTCCAATATCCCTTC	45.0	50.0	58.1	57.4	838-945 bp	1.0	75.3