Hotspot

trnR-ACG-trnN-GUU

IRb · 104085-104614 · 530 bp

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Pi 0.0151

MarkerSeek score 65.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 530
variable_sites: 8
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	671-707 bp	1.0	79.3
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	622-658 bp	1.0	78.1
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	626-662 bp	1.0	77.3
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TCTCCCGTTGTTCCTCAGTA	55.0	50.0	57.8	57.7	675-711 bp	1.0	76.9
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	665-701 bp	1.0	76.8