Hotspot

rpl2-rpl23

LSC · 81931-81948 · 18 bp

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Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	ACTGTCTACGGTTCCATTGC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.9	58.3	128 bp	1.0	77.7
rpl2-rpl23_p2	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	105 bp	1.0	77.2
rpl2-rpl23_p3	ACTGTCTACGGTTCCATTGC	ATCATTACGCTTCAACCGGG	50.0	50.0	57.9	58.3	135 bp	1.0	77.1
rpl2-rpl23_p4	ACTGTCTACGGTTCCATTGC	TCCGGGAAAGGGTAGAAGAA	50.0	50.0	57.9	57.7	196 bp	1.0	77.0
rpl2-rpl23_p5	ACTGTCTACGGTTCCATTGC	TTCCGGGAAAGGGTAGAAGA	50.0	50.0	57.9	57.7	197 bp	1.0	77.0