Hotspot

psbC-trnS-UGA

LSC · 33323-33548 · 226 bp

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Pi 0.0222

MarkerSeek score 65.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 5
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	428-495 bp	1.0	79.3
psbC-trnS-UGA_p2	TCGTAGGTCATTTGTGGCAC	GTCTTTCGGAGAGATGGCTG	50.0	55.0	58.2	58.1	436-503 bp	1.0	78.9
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.2	57.9	408-475 bp	1.0	78.5
psbC-trnS-UGA_p4	TCGTAGGTCATTTGTGGCAC	AGTCTTTCGGAGAGATGGCT	50.0	50.0	58.2	58.1	437-504 bp	1.0	78.3
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	TCTTTCGGAGAGATGGCTGA	50.0	50.0	58.2	58.1	435-502 bp	1.0	78.3