Hotspot

trnT-GGU-psbD

LSC · 29678-30891 · 1214 bp

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Pi 0.00741

MarkerSeek score 63.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 9
pi: 0.00741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1370-1458 bp	1.0	79.7
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1388-1476 bp	1.0	78.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1333-1421 bp	1.0	78.2
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1378-1466 bp	1.0	77.7
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1351-1439 bp	1.0	77.3