Hotspot

trnK-UUU-rps16

LSC · 2547-3214 · 668 bp

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Pi 0.0165

MarkerSeek score 57.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 11
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GTGGGGGTTTTTAAGGAAC	50.0	47.4	58.2	53.7	750-911 bp	1.0	41.3
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GTGGGGGTTTTTAAGGAAC	50.0	47.4	58.2	53.7	749-910 bp	1.0	41.3
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	GTGGGGGTTTTTAAGGAAC	55.0	47.4	57.9	53.7	811-972 bp	1.0	41.2
trnK-UUU-rps16_p4	TCAGTCGTGGTCTTCCAAAC	GTGGGGGTTTTTAAGGAAC	50.0	47.4	57.8	53.7	835-996 bp	1.0	41.1
trnK-UUU-rps16_p5	CAGTCGTGGTCTTCCAAACT	GTGGGGGTTTTTAAGGAAC	50.0	47.4	57.8	53.7	834-995 bp	1.0	41.1