Hotspot

ycf1

SSC · 123719-129187 · 5469 bp

Pi 0.0317

MarkerSeek score 59.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 173
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACTCATCGAAGTCCTCAGC	CGGGTTCAAAGGCGTAAAAC	55.0	50.0	58.1	58.0	413 bp	1.0	84.5
ycf1_p2	GACAGAACCAAAGGGAAGGG	CGGGTTCAAAGGCGTAAAAC	55.0	50.0	58.1	58.0	657 bp	1.0	83.2
ycf1_p3	GGACAGAACCAAAGGGAAGG	CGGGTTCAAAGGCGTAAAAC	55.0	50.0	58.1	58.0	658 bp	1.0	83.2
ycf1_p4	TCCGCGATTTTTATCCACGT	CGGGTTCAAAGGCGTAAAAC	45.0	50.0	58.0	58.0	248 bp	0.5	59.6
ycf1_p5	TCCGCGATTTTTATCCACGT	ATGGGTTCTATCGGGGAACA	45.0	50.0	58.0	58.1	874 bp	0.5	59.0