Hotspot

rpl32-trnL-UAG

SSC · 113529-114098 · 570 bp

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Pi 0.0673
MarkerSeek score 78.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
570
variable_sites
36
pi
0.0673
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 694-1009 bp 1.0 85.8
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 695-1010 bp 1.0 85.8
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 715-1030 bp 1.0 85.1
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 717-1032 bp 1.0 85.1
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 704-1019 bp 1.0 84.8