Hotspot

ndhF-rpl32

SSC · 112425-113366 · 942 bp

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Pi 0.0753
MarkerSeek score 88.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
942
variable_sites
68
pi
0.0753
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
88.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG TAAGGCTGCCCAATATCCCT 50.0 50.0 58.0 58.2 1116-1127 bp 1.0 86.0
ndhF-rpl32_p2 TGCCGACGGAAAAAGAAGAA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1114-1125 bp 1.0 86.0
ndhF-rpl32_p3 TTGCCGACGGAAAAAGAAGA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1115-1126 bp 1.0 86.0
ndhF-rpl32_p4 AAAAGAAGAAGTCCCGCTCC TAAGGCTGCCCAATATCCCT 50.0 50.0 57.8 58.2 1104-1115 bp 1.0 84.9
ndhF-rpl32_p5 GTTGCCGACGGAAAAAGAAG GACAACGCTTTTAAGGCTGC 50.0 50.0 58.0 58.3 1127-1138 bp 1.0 84.8