Hotspot

ndhC-trnV-UAC

LSC · 50719-51790 · 1072 bp

Back to Apium

Pi 0.0619
MarkerSeek score 66.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1072
variable_sites
36
pi
0.0619
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.54
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 731-1131 bp 1.0 47.1
ndhC-trnV-UAC_p2 ATCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 30.4 55.0 54.5 58.1 732-1132 bp 1.0 47.1
ndhC-trnV-UAC_p3 TCATATTCGTAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 30.4 55.0 54.5 58.1 731-1131 bp 1.0 47.1
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 716-1116 bp 1.0 47.1
ndhC-trnV-UAC_p5 CATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 33.3 55.0 52.3 58.1 730-1130 bp 1.0 47.1