Hotspot

ycf3-trnS-GGA

LSC · 44539-45374 · 836 bp

Back to Apium

Pi 0.0364
MarkerSeek score 76.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
836
variable_sites
29
pi
0.0364
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 918-925 bp 1.0 76.9
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 939-946 bp 1.0 76.3
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 47.6 45.0 58.0 57.8 889-896 bp 1.0 76.3
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 47.6 50.0 58.0 58.3 891-898 bp 1.0 75.8
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 919-926 bp 1.0 74.6