Hotspot

trnT-GGU-psbD

LSC · 31841-32830 · 990 bp

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Pi 0.0555
MarkerSeek score 78.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
990
variable_sites
54
pi
0.0555
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC TGGTAAATTTCCCAAGGGCT 50.0 45.0 58.1 57.0 1095-1552 bp 1.0 75.6
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA TGGTAAATTTCCCAAGGGCT 50.0 45.0 57.9 57.0 1091-1548 bp 1.0 75.4
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TGGTAAATTTCCCAAGGGCT 50.0 45.0 58.3 57.0 1054-1511 bp 1.0 74.1
trnT-GGU-psbD_p4 AAGGGCCCTTTTAACTCAGC TTGGTAAATTTCCCAAGGGCT 50.0 42.9 58.1 57.7 1096-1553 bp 1.0 73.5
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA TTGGTAAATTTCCCAAGGGCT 50.0 42.9 57.9 57.7 1092-1549 bp 1.0 73.3