Hotspot

trnT-CGU-trnR-UCU

LSC · 9993-10127 · 135 bp

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Pi 0.108
MarkerSeek score 80.40
Primer available yes
Label trnT-CGU-trnR-UCU

Feature Metadata

label_name
trnT-CGU-trnR-UCU
feature_type
igs
region
LSC
length_bp
135
variable_sites
13
pi
0.108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-CGU-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 201-216 bp 1.0 72.3
trnT-CGU-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 204-219 bp 1.0 72.0
trnT-CGU-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 207-222 bp 1.0 71.5
trnT-CGU-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 210-225 bp 1.0 71.2
trnT-CGU-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 205-220 bp 1.0 69.8