Hotspot

trnS-GCU-trnT-CGU

LSC · 8686-9229 · 544 bp

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Pi 0.0672
MarkerSeek score 80.50
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
544
variable_sites
34
pi
0.0672
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 ATTAGCAATCCGCCGCTTTA ACCACTAAACTATACCCGCT 45.0 45.0 58.0 55.6 574-604 bp 1.0 69.5
trnS-GCU-trnT-CGU_p2 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 623-653 bp 1.0 68.2
trnS-GCU-trnT-CGU_p3 ATTAGCAATCCGCCGCTTTA ACTCGAACGAATCACACTTT 45.0 40.0 58.0 55.4 595-625 bp 1.0 67.0
trnS-GCU-trnT-CGU_p4 GCTTTAGTCCACTCAGCCAT ACCACTAAACTATACCCGCT 50.0 45.0 57.6 55.6 560-590 bp 1.0 66.6
trnS-GCU-trnT-CGU_p5 ATTAGCAATCCGCCGCTTTA CGAACGAATCACACTTTTACCA 45.0 40.9 58.0 57.3 592-622 bp 1.0 66.4