Pi
0.394
MarkerSeek score
73.70
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | GAGCTACTGAGGAACAACGG | CTTTATTCGTTGGGGCCGTA | 55.0 | 50.0 | 58.0 | 58.0 | 1389 bp | 0.5 | 59.8 |
| ycf1_p2 | GAGCTACTGAGGAACAACGG | TACTTTATTCGTTGGGGCCG | 55.0 | 50.0 | 58.0 | 58.0 | 1391 bp | 0.5 | 59.8 |
| ycf1_p3 | GAGCTACTGAGGAACAACGG | TTTATTCGTTGGGGCCGTAG | 55.0 | 50.0 | 58.0 | 58.0 | 1388 bp | 0.5 | 59.8 |
| ycf1_p4 | GGTTAAATAGCCCCGGACTG | CTTTATTCGTTGGGGCCGTA | 55.0 | 50.0 | 58.0 | 58.0 | 1654 bp | 0.5 | 59.5 |
| ycf1_p5 | GGTTAAATAGCCCCGGACTG | TTTATTCGTTGGGGCCGTAG | 55.0 | 50.0 | 58.0 | 58.0 | 1653 bp | 0.5 | 59.5 |
| ycf1_p1 | TTCTTTTCCCTGCCCAATCC | TCTTCGAATGACAACACGCA | 50.0 | 45.0 | 58.1 | 58.1 | 491 bp | 0.5 | 58.4 |
| ycf1_p2 | CTACGACGCCTAGGGGATAA | TCTTCGAATGACAACACGCA | 55.0 | 45.0 | 58.1 | 58.1 | 1037 bp | 0.5 | 58.1 |
| ycf1_p3 | TCCTTAAGCCCGGAAGAGTT | TCTTCGAATGACAACACGCA | 50.0 | 45.0 | 58.3 | 58.1 | 754 bp | 0.5 | 56.1 |
| ycf1_p4 | TTCCTTAAGCCCGGAAGAGT | TCTTCGAATGACAACACGCA | 50.0 | 45.0 | 58.3 | 58.1 | 755 bp | 0.5 | 56.1 |
| ycf1_p5 | CCATTCTTTTCCCTGCCCAA | TCTTCGAATGACAACACGCA | 50.0 | 45.0 | 58.4 | 58.1 | 494 bp | 0.5 | 56.0 |