Hotspot

ndhI-ndhA

SSC · 115759-115839 · 81 bp

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Pi 0.452
MarkerSeek score 76.00
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
81
variable_sites
33
pi
0.452
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 CGGCCCAAAGCAATTTGATT TGATTTACCGGAAGCGGAAG 45.0 50.0 57.9 58.0 912 bp 0.5 59.1
ndhI-ndhA_p2 CGTGGCGATCATAAGTCGAA TGATTTACCGGAAGCGGAAG 50.0 50.0 58.2 58.0 881 bp 0.5 58.5
ndhI-ndhA_p3 TTCGTGGCGATCATAAGTCG TGATTTACCGGAAGCGGAAG 50.0 50.0 58.2 58.0 883 bp 0.5 58.5
ndhI-ndhA_p4 CGGCCCAAAGCAATTTGATT TTTACCGGAAGCGGAAGAAG 45.0 50.0 57.9 57.9 909 bp 0.5 58.5
ndhI-ndhA_p5 ACCAATGTACCTTGCAGCTC TGATTTACCGGAAGCGGAAG 50.0 50.0 58.2 58.0 550 bp 0.5 58.5