Hotspot

psbK-trnQ-UUG

LSC · 54841-55287 · 447 bp

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Pi 0.0427
MarkerSeek score 76.80
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
447
variable_sites
19
pi
0.0427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 TAGGCTTCGGGCAATTTAGC GACCAAAACCCGTTGCCTTA 50.0 50.0 58.3 58.7 574-583 bp 1.0 74.9
psbK-trnQ-UUG_p2 CGTAGGCTTCGGGCAATTTA GACCAAAACCCGTTGCCTTA 50.0 50.0 58.3 58.7 576-585 bp 1.0 74.7
psbK-trnQ-UUG_p3 TAGGCTTCGGGCAATTTAGC GAATAGCGGGACCAAAACCC 50.0 55.0 58.3 58.9 583-592 bp 1.0 73.1
psbK-trnQ-UUG_p4 CGTAGGCTTCGGGCAATTTA GAATAGCGGGACCAAAACCC 50.0 55.0 58.3 58.9 585-594 bp 1.0 72.9
psbK-trnQ-UUG_p5 TAGGCTTCGGGCAATTTAGC ACCAAAACCCGTTGCCTTAC 50.0 50.0 58.3 59.0 573-582 bp 1.0 72.6