Hotspot

trnR-UCU-trnG-GCC

LSC · 52315-52578 · 264 bp

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Pi 0.044
MarkerSeek score 76.90
Primer available yes
Label trnR-UCU-trnG-GCC

Feature Metadata

label_name
trnR-UCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
264
variable_sites
11
pi
0.044
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-GCC_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 338-344 bp 1.0 69.2
trnR-UCU-trnG-GCC_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 341-347 bp 1.0 68.9
trnR-UCU-trnG-GCC_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 344-350 bp 1.0 68.4
trnR-UCU-trnG-GCC_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 347-353 bp 1.0 68.2
trnR-UCU-trnG-GCC_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 342-348 bp 1.0 66.7