Hotspot

PS16_p039

SSC · 51576-107691 · 56116 bp

Back to Aphyllon

Pi 0.0696

MarkerSeek score 75.10

Primer available yes

Label PS16_p039

Feature Metadata

label_name: PS16_p039
feature_type: gene
region: SSC
length_bp: 56116
variable_sites: 4865
pi: 0.0696
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PS16_p039_p1	GCTTGGCGATTCTAGAACCA	TTGTACGGGATGTAACCCCT	50.0	50.0	58.0	58.0	641-1640 bp	0.666667	75.8
PS16_p039_p2	GCTTGGCGATTCTAGAACCA	AGAGACGCAGCATTAAGAGC	50.0	50.0	58.0	58.1	690-1689 bp	0.666667	75.4
PS16_p039_p3	GCTTGGCGATTCTAGAACCA	GAGACGCAGCATTAAGAGCT	50.0	50.0	58.0	58.1	689-1688 bp	0.666667	75.4
PS16_p039_p4	GCTTGGCGATTCTAGAACCA	AAAAGGTCCCGGTCTAGGAA	50.0	50.0	58.0	58.0	1708 bp	0.333333	52.9
PS16_p039_p5	GGTAAACCAGCGGATCCATT	AAAAGGTCCCGGTCTAGGAA	50.0	50.0	57.9	58.0	1679 bp	0.333333	52.6