Hotspot

clpP-psbB

LSC · 53775-54251 · 477 bp

Back to Aphyllon

Pi 0.13

MarkerSeek score 80.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 85
pi: 0.13
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1052 bp	0.333333	53.2
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CCCGCCTGTAATACTCCAAC	50.0	55.0	58.0	58.1	796 bp	0.333333	52.9
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CCGCCTGTAATACTCCAACC	50.0	55.0	58.0	58.1	795 bp	0.333333	52.9
clpP-psbB_p4	TTTATCTTTCGGGGGCGAAG	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1137 bp	0.333333	52.9
clpP-psbB_p5	CAAATCGCGGTGGTCTTTTC	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1499 bp	0.333333	52.9