Hotspot

matK-trnK-UUU

LSC · 3696-4397 · 702 bp

Back to Aphyllon

Pi 0.0924

MarkerSeek score 81.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 95
pi: 0.0924
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCACGATCATGAGCAAATGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	832-833 bp	0.666667	73.2
matK-trnK-UUU_p2	CCACGATCATGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	831-832 bp	0.666667	73.2
matK-trnK-UUU_p3	GATTTCCGCATGGACAGAGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	1212 bp	0.333333	51.7
matK-trnK-UUU_p4	GATTTCCGCATGGACAGAGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	1211 bp	0.333333	51.7
matK-trnK-UUU_p5	GATTTCCGCATGGACAGAGT	TCGCGGTTTTATTAGCTCGT	50.0	45.0	58.0	57.7	1364 bp	0.333333	50.8