Hotspot

ndhD

SSC · 114448-115674 · 1227 bp

Back to Aphragmus

Pi 0.239

MarkerSeek score 80.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1227
variable_sites: 250
pi: 0.239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCAATTGTGGCTTCGAT	TGTGAATCTGCCTGTCCAAC	45.0	50.0	58.2	58.1	1929-2067 bp	1.0	89.8
ndhD_p2	TCTGCAATTGTGGCTTCGAT	TGAGTACGCGTTCTTTGGAC	45.0	50.0	58.2	58.2	1740-1869 bp	1.0	88.8
ndhD_p3	TCTGCAATTGTGGCTTCGAT	AAGAACCGAGGACTGTGTTG	45.0	50.0	58.2	57.8	1960-2098 bp	1.0	88.7
ndhD_p4	TCTGCAATTGTGGCTTCGAT	AACTCGCATCAACCAATCGA	45.0	45.0	58.2	57.9	2508 bp	0.5	57.9
ndhD_p5	TCTGCAATTGTGGCTTCGAT	CAACTCGCATCAACCAATCG	45.0	50.0	58.2	57.8	2509 bp	0.5	57.3