Hotspot

rpl32-trnL-CUA

SSC · 111995-112755 · 761 bp

Back to Aphragmus

Pi 0.069
MarkerSeek score 75.30
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
761
variable_sites
44
pi
0.069
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GCACTCTCGTACGGATTTCA TAACCACTGGGACTGGAGAG 50.0 55.0 57.7 58.1 1740-1742 bp 1.0 84.6
rpl32-trnL-CUA_p2 AAATGAGCACTCTCGTACGG TAACCACTGGGACTGGAGAG 50.0 55.0 57.7 58.1 1746-1748 bp 1.0 84.6
rpl32-trnL-CUA_p3 GCACTCTCGTACGGATTTCA TTTCCAAAAAGCAGGTCCGA 50.0 45.0 57.7 57.9 1854-1856 bp 1.0 84.5
rpl32-trnL-CUA_p4 AAATGAGCACTCTCGTACGG TTTCCAAAAAGCAGGTCCGA 50.0 45.0 57.7 57.9 1860-1862 bp 1.0 84.5
rpl32-trnL-CUA_p5 GCACTCTCGTACGGATTTCA GAGCGCCGTAGCCTAATATC 50.0 55.0 57.7 58.1 1780-1782 bp 1.0 84.4