Hotspot

ndhC-trnV-GUA

LSC · 49016-49677 · 662 bp

Back to Aphragmus

Pi 0.0619

MarkerSeek score 74.10

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 41
pi: 0.0619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	GGCTGGAAAGTCTTGACCAA	ACCTACGTCAAGAGAACCCA	50.0	50.0	58.0	58.0	1465-1516 bp	1.0	83.0
ndhC-trnV-GUA_p2	GTACCATAAAGAAGCGGCCA	ACCTACGTCAAGAGAACCCA	50.0	50.0	58.0	58.0	1487 bp	0.5	59.7
ndhC-trnV-GUA_p3	TGGTACCATAAAGAAGCGGC	ACCTACGTCAAGAGAACCCA	50.0	50.0	58.0	58.0	1489 bp	0.5	59.7
ndhC-trnV-GUA_p4	CATTGCCCACGGATACAGAA	ACCTACGTCAAGAGAACCCA	50.0	50.0	58.0	58.0	1194 bp	0.5	59.7
ndhC-trnV-GUA_p5	AACTCATTGCCCACGGATAC	ACCTACGTCAAGAGAACCCA	50.0	50.0	58.0	58.0	1198 bp	0.5	59.6