Hotspot

trnT-ACC-psbD

LSC · 30661-31897 · 1237 bp

Back to Aphragmus

Pi 0.0472
MarkerSeek score 76.90
Primer available yes
Label trnT-ACC-psbD

Feature Metadata

label_name
trnT-ACC-psbD
feature_type
igs
region
LSC
length_bp
1237
variable_sites
56
pi
0.0472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACC-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1274-1316 bp 1.0 46.6
trnT-ACC-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1277-1319 bp 1.0 46.6
trnT-ACC-psbD_p3 CCAAGACGGCCCTTTTAACT TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1296-1338 bp 1.0 46.5
trnT-ACC-psbD_p4 ACGGCCCTTTTAACTCAGTG TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1291-1333 bp 1.0 46.5
trnT-ACC-psbD_p5 GACGGCCCTTTTAACTCAGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.8 53.0 1292-1334 bp 1.0 46.5