Hotspot

petN-psbM

LSC · 27937-28477 · 541 bp

Back to Aphragmus

Pi 0.0598

MarkerSeek score 78.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 32
pi: 0.0598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	58.0	58.0	642-644 bp	1.0	76.4
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	GCACTCTTCATTCTCGTTCCT	55.0	47.6	57.9	58.0	641-643 bp	1.0	75.8
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	ACTGCACTCTTCATTCTCGT	50.0	45.0	58.0	56.9	645-647 bp	1.0	75.7
petN-psbM_p4	CGTAGTGTGGGGAAGAAGTG	ACTGCACTCTTCATTCTCGT	55.0	45.0	57.9	56.9	644-646 bp	1.0	75.1
petN-psbM_p5	CTCTCGTAGTGTGGGGAAGA	GCACTCTTCATTCTCGTTCCT	55.0	47.6	58.2	58.0	645-647 bp	1.0	75.1