Hotspot

trnG-GGA-trnR-AGA

LSC · 9181-9327 · 147 bp

Back to Aphragmus

Pi 0.128
MarkerSeek score 81.50
Primer available yes
Label trnG-GGA-trnR-AGA

Feature Metadata

label_name
trnG-GGA-trnR-AGA
feature_type
igs
region
LSC
length_bp
147
variable_sites
18
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGA-trnR-AGA_p1 AGCCTTCCAAGCTAACGATG TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 269 bp 1.0 54.3
trnG-GGA-trnR-AGA_p2 ACCCTTAGCCTTCCAAGCTA TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 275 bp 1.0 54.1
trnG-GGA-trnR-AGA_p3 AAACCCTTAGCCTTCCAAGC TGATATTGCGTCCAATAGGA 50.0 40.0 58.1 53.9 277 bp 1.0 53.7
trnG-GGA-trnR-AGA_p4 CGACGTCGACTAAAACCCTT TGATATTGCGTCCAATAGGA 50.0 40.0 57.9 53.9 289 bp 1.0 53.6
trnG-GGA-trnR-AGA_p5 AGCCTTCCAAGCTAACGATG TTGATATTGCGTCCAATAGGA 50.0 38.1 58.0 54.8 270 bp 1.0 53.3