Hotspot

trnH-CAC-psbA

LSC · 75-291 · 217 bp

Back to Aphragmus

Pi 0.106
MarkerSeek score 86.90
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
217
variable_sites
22
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 275-278 bp 1.0 87.5
trnH-CAC-psbA_p2 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 276-279 bp 1.0 87.2
trnH-CAC-psbA_p3 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 290-293 bp 1.0 86.9
trnH-CAC-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 293-296 bp 1.0 86.7
trnH-CAC-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 291-294 bp 1.0 86.7