Hotspot

rpoC1

Genome · 40143-46733 · 6591 bp

Back to Aphanochaete

Pi 0.412

MarkerSeek score 73.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 6591
variable_sites: 2208
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GCTATTTGCGTTTTATGCCGA	GAACGCATTTTTGGCCCTAC	42.9	50.0	57.9	58.0	725 bp	0.5	51.4
rpoC1_p2	GCTATTTGCGTTTTATGCCGA	AATGCCAATACCTTGCACCA	42.9	45.0	57.9	58.1	782 bp	0.5	51.0
rpoC1_p3	GCTATTTGCGTTTTATGCCGA	AACGCGTCCAGATTTGATGA	42.9	45.0	57.9	57.9	899 bp	0.5	50.8
rpoC1_p4	GCTATTTGCGTTTTATGCCGA	ACGCGTCCAGATTTGATGAA	42.9	45.0	57.9	57.9	898 bp	0.5	50.8
rpoC1_p5	GCTATTTGCGTTTTATGCCGA	TCTGTGAACGCATTTTTGGC	42.9	45.0	57.9	57.9	730 bp	0.5	50.5