Hotspot

ftsH

Genome · 106639-117591 · 10953 bp

Back to Aphanochaete

Pi 0.293

MarkerSeek score 77.00

Primer available yes

Label ftsH

Feature Metadata

label_name: ftsH
feature_type: gene
region: Genome
length_bp: 10953
variable_sites: 3010
pi: 0.293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH_p1	AGATTGGCATTCAAACGACT	AACGCGGTAACTTTGATGGA	40.0	45.0	55.6	57.8	1043 bp	0.5	39.8
ftsH_p2	AGATTGGCATTCAAACGACTT	AACGCGGTAACTTTGATGGA	38.1	45.0	56.4	57.8	1043 bp	0.5	38.1
ftsH_p3	AGATTGGCATTCAAACGACT	ACGCGGTAACTTTGATGGAT	40.0	45.0	55.6	57.6	1042 bp	0.5	38.0
ftsH_p4	TGTAGATTGGCATTCAAACGAC	AACGCGGTAACTTTGATGGA	40.9	45.0	57.3	57.8	1046 bp	0.5	37.2
ftsH_p5	ACTGTAGATTGGCATTCAAACG	AACGCGGTAACTTTGATGGA	40.9	45.0	57.3	57.8	1048 bp	0.5	37.1