Hotspot

chlN

Genome · 83796-85313 · 1518 bp

Back to Aphanochaete

Pi 0.17

MarkerSeek score 83.00

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1518
variable_sites: 246
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	AGGAATGTGAACATTGGTGCT	ATTCGTAAACTCGGCTCAGC	42.9	50.0	58.1	58.4	2896 bp	0.5	48.2
chlN_p2	AGGAATGTGAACATTGGTGCT	ACAAGACGCCACTAATTCGT	42.9	45.0	58.1	57.5	2828 bp	0.5	47.4
chlN_p3	GAGGAATGTGAACATTGGTGC	ATTCGTAAACTCGGCTCAGC	47.6	50.0	57.8	58.4	2897 bp	0.5	47.4
chlN_p4	AGGAATGTGAACATTGGTGCT	CGGAGCTGAGCCTATTTCAT	42.9	50.0	58.1	57.5	2877 bp	0.5	46.7
chlN_p5	GAGGAATGTGAACATTGGTGC	ACAAGACGCCACTAATTCGT	47.6	45.0	57.8	57.5	2829 bp	0.5	46.6