Hotspot

rpoBb-rpl32

Genome · 133015-133617 · 603 bp

Back to Aphanochaete

Pi 0.326

MarkerSeek score 83.00

Primer available yes

Label rpoBb-rpl32

Feature Metadata

label_name: rpoBb-rpl32
feature_type: igs
region: Genome
length_bp: 603
variable_sites: 181
pi: 0.326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-rpl32_p1	AAATCCGACGATGTGACAGG	ATAAAGAGTGGCTGGGAGAC	50.0	50.0	58.0	56.6	1530 bp	0.5	48.9
rpoBb-rpl32_p2	ATAGGGACACCAGCCTCTTT	ATAAAGAGTGGCTGGGAGAC	50.0	50.0	58.0	56.6	1464 bp	0.5	48.7
rpoBb-rpl32_p3	TGGGCAACGTTTAGGTGAAA	ATAAAGAGTGGCTGGGAGAC	45.0	50.0	57.9	56.6	1612 bp	0.5	48.5
rpoBb-rpl32_p4	GTGAAATGGAAGTTTGGGCG	ATAAAGAGTGGCTGGGAGAC	50.0	50.0	57.9	56.6	1598 bp	0.5	48.4
rpoBb-rpl32_p5	ACCTGCAGGATCTGTCACTA	ATAAAGAGTGGCTGGGAGAC	50.0	50.0	57.8	56.6	1912 bp	0.5	47.0