Hotspot

petB-psbI

Genome · 81906-82567 · 662 bp

Back to Aphanochaete

Pi 0.341

MarkerSeek score 83.40

Primer available yes

Label petB-psbI

Feature Metadata

label_name: petB-psbI
feature_type: igs
region: Genome
length_bp: 662
variable_sites: 210
pi: 0.341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-psbI_p1	TTTTACGAGGGGGTGTAGGT	AGCACCAATGTTCACATTCCT	50.0	42.9	58.0	58.1	1314 bp	0.5	50.8
petB-psbI_p2	CCGTTGGTCTGCAAGTATGA	AGCACCAATGTTCACATTCCT	50.0	42.9	57.9	58.1	1559 bp	0.5	50.3
petB-psbI_p3	CTATTCACCGTTGGTCTGCA	AGCACCAATGTTCACATTCCT	50.0	42.9	57.9	58.1	1566 bp	0.5	50.3
petB-psbI_p4	GCAAGGTATTTCTGGTCCGT	AGCACCAATGTTCACATTCCT	50.0	42.9	57.9	58.1	1193 bp	0.5	50.2
petB-psbI_p5	TCGTTACCATGGGATCAGGT	AGCACCAATGTTCACATTCCT	50.0	42.9	58.1	58.1	1408 bp	0.5	50.0