Hotspot

trnS(gga)-rrs

Genome · 61911-62518 · 608 bp

Back to Aphanochaete

Pi 0.339

MarkerSeek score 82.30

Primer available yes

Label trnS(gga)-rrs

Feature Metadata

label_name: trnS(gga)-rrs
feature_type: igs
region: Genome
length_bp: 608
variable_sites: 200
pi: 0.339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gga)-rrs_p1	TCGAACCCTCGGTAGTTCTT	CTCTCAGTGTCAGTAACGGC	50.0	55.0	58.1	58.0	1419 bp	0.5	59.2
trnS(gga)-rrs_p2	TTCGAACCCTCGGTAGTTCT	CTCTCAGTGTCAGTAACGGC	50.0	55.0	58.1	58.0	1420 bp	0.5	59.2
trnS(gga)-rrs_p3	TTCAAAGCGAGCTTCCCATT	CTCTCAGTGTCAGTAACGGC	45.0	55.0	58.1	58.0	2063 bp	0.5	59.1
trnS(gga)-rrs_p4	TTTCAAAGCGAGCTTCCCAT	CTCTCAGTGTCAGTAACGGC	45.0	55.0	58.1	58.0	2064 bp	0.5	59.1
trnS(gga)-rrs_p5	TCGAACCCTCGGTAGTTCTT	TACGTCGTCTGACTTGCATG	50.0	50.0	58.1	58.0	749 bp	0.5	59.1