Hotspot

trnM(cau)-trnfM(cau)

Genome · 48217-49561 · 1345 bp

Back to Aphanochaete

Pi 0.412

MarkerSeek score 78.20

Primer available yes

Label trnM(cau)-trnfM(cau)

Feature Metadata

label_name: trnM(cau)-trnfM(cau)
feature_type: igs
region: Genome
length_bp: 1345
variable_sites: 419
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM(cau)-trnfM(cau)_p1	CGGTTTAGGCAATCGACTCA	ATATCGCTGGGTAGAGCAGT	50.0	50.0	58.0	58.0	1481 bp	0.5	59.9
trnM(cau)-trnfM(cau)_p2	CGGTTTAGGCAATCGACTCA	AGCCTGCTTAACTCAATCGG	50.0	50.0	58.0	58.0	1714 bp	0.5	59.7
trnM(cau)-trnfM(cau)_p3	CGGTTTAGGCAATCGACTCA	TTTCCAAAACCGGATGTCGT	50.0	45.0	58.0	58.0	2026 bp	0.5	59.7
trnM(cau)-trnfM(cau)_p4	CGGTTTAGGCAATCGACTCA	TAGAACGCCGGTTTCCAAAA	50.0	45.0	58.0	58.0	2037 bp	0.5	59.7
trnM(cau)-trnfM(cau)_p5	ACCCATTGCCTTACCACTTG	ATATCGCTGGGTAGAGCAGT	50.0	50.0	58.1	58.0	2119 bp	0.5	59.4