Hotspot

rps3

LSC · 86592-87254 · 663 bp

Back to Aphanocalyx

Pi 0.00603

MarkerSeek score 51.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 4
pi: 0.00603
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCGCACACTAAGCATAGCAA	ACCCAGATAGGACGTGTCAT	45.0	50.0	57.6	57.8	835 bp	0.5	55.7
rps3_p2	TCGCACACTAAGCATAGCAA	ACACCCAGATAGGACGTGTC	45.0	55.0	57.6	58.8	837 bp	0.5	50.4
rps3_p3	TCGCACACTAAGCATAGCAA	CACCCAGATAGGACGTGTCA	45.0	55.0	57.6	58.8	836 bp	0.5	50.4
rps3_p4	TCGCACACTAAGCATAGCAAT	ACCCAGATAGGACGTGTCAT	42.9	50.0	58.1	57.8	835 bp	0.5	50.0
rps3_p5	CGCACACTAAGCATAGCAAT	ACCCAGATAGGACGTGTCAT	45.0	50.0	56.6	57.8	834 bp	0.5	47.5