Hotspot

trnN-GUU-trnR-ACG

IRa · 134801-135479 · 679 bp

Back to Aphanocalyx

Pi 0.0151
MarkerSeek score 70.00
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
679
variable_sites
10
pi
0.0151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 843-861 bp 1.0 80.1
trnN-GUU-trnR-ACG_p2 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 766-784 bp 1.0 80.0
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 753-771 bp 1.0 80.0
trnN-GUU-trnR-ACG_p4 TGGGTTGAGAACGGGAATTG GAACAGATCCAGTGGAGACG 50.0 55.0 58.1 57.7 880-898 bp 1.0 79.5
trnN-GUU-trnR-ACG_p5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 842-860 bp 1.0 79.4