Hotspot

ndhI-ndhA

SSC · 124557-124632 · 76 bp

Back to Aphanocalyx

Pi 0.0263

MarkerSeek score 67.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 76
variable_sites: 2
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	ACAACTTCGTCTCAACTGCT	45.0	45.0	58.1	57.4	163 bp	1.0	75.7
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	ACAACTTCGTCTCAACTGCT	45.0	45.0	58.1	57.4	164 bp	1.0	75.7
ndhI-ndhA_p3	ACGATTTGCATGGGATAGGG	ACAACTTCGTCTCAACTGCT	50.0	45.0	57.7	57.4	211 bp	1.0	73.7
ndhI-ndhA_p4	CGATTTGCATGGGATAGGGT	ACAACTTCGTCTCAACTGCT	50.0	45.0	57.7	57.4	210 bp	1.0	73.7
ndhI-ndhA_p5	CAGGGAAACGATTTGCATGG	ACAACTTCGTCTCAACTGCT	50.0	45.0	57.7	57.4	218 bp	1.0	73.6