Hotspot

ndhG-ndhI

SSC · 123577-124058 · 482 bp

Back to Aphanocalyx

Pi 0.0145

MarkerSeek score 51.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 482
variable_sites: 7
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATCGGTTCAGGCAAATCCAT	TCGAACAATTCTAAACTCGCCT	45.0	40.9	57.6	58.1	536-549 bp	1.0	60.9
ndhG-ndhI_p2	ATCGGTTCAGGCAAATCCAT	CGAACAATTCTAAACTCGCCTC	45.0	45.5	57.6	57.8	535-548 bp	1.0	59.5
ndhG-ndhI_p3	ATCGGTTCAGGCAAATCCAT	TCGAACAATTCTAAACTCGCC	45.0	42.9	57.6	56.7	536-549 bp	1.0	58.7
ndhG-ndhI_p4	ATCGGTTCAGGCAAATCCAT	CGAACAATTCTAAACTCGCCT	45.0	42.9	57.6	56.6	535-548 bp	1.0	58.7
ndhG-ndhI_p5	ATCGGTTCAGGCAAATCCAT	TTCGAACAATTCTAAACTCGCC	45.0	40.9	57.6	57.3	537-550 bp	1.0	56.2