Hotspot

rps3-rps19

LSC · 87255-87900 · 646 bp

Back to Aphanocalyx

Pi 0.014

MarkerSeek score 61.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 9
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACCAAGTCTGAAACCGAGTG	TCTAAACTTTCGGGGACACG	50.0	50.0	57.8	57.6	734-818 bp	1.0	75.9
rps3-rps19_p2	CCAAGTCTGAAACCGAGTGG	TCTAAACTTTCGGGGACACG	55.0	50.0	58.5	57.6	733-817 bp	1.0	73.9
rps3-rps19_p3	CAAGTCTGAAACCGAGTGGA	TCTAAACTTTCGGGGACACG	50.0	50.0	57.5	57.6	732-816 bp	1.0	73.6
rps3-rps19_p4	ACCAAGTCTGAAACCGAGTG	GGGACACGCCAAAAACGATA	50.0	50.0	57.8	58.8	722-806 bp	1.0	72.7
rps3-rps19_p5	ACCAAGTCTGAAACCGAGTG	GGACACGCCAAAAACGATAA	50.0	45.0	57.8	57.0	721-805 bp	1.0	71.7