Hotspot

rbcL-accD

LSC · 59935-60915 · 981 bp

Back to Aphanocalyx

Pi 0.0227

MarkerSeek score 71.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 22
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TTAGTTAGCCCACACCCGTA	50.0	50.0	58.0	58.1	1124-1160 bp	1.0	81.6
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTAGTTAGCCCACACCCGTA	55.0	50.0	58.0	58.1	1139-1175 bp	1.0	81.5
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	TAGTTAGCCCACACCCGTAT	50.0	50.0	58.0	57.8	1123-1159 bp	1.0	80.8
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TAGTTAGCCCACACCCGTAT	55.0	50.0	58.0	57.8	1138-1174 bp	1.0	80.7
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	GCCCACACCCGTATTCTAAC	50.0	55.0	58.0	58.3	1117-1153 bp	1.0	79.4