Hotspot

ndhC-trnV-UAC

LSC · 54029-54639 · 611 bp

Back to Aphanocalyx

Pi 0.00565

MarkerSeek score 64.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 3
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	682-761 bp	1.0	77.2
ndhC-trnV-UAC_p2	TCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	683-762 bp	1.0	77.0
ndhC-trnV-UAC_p3	TTTCTCTGGCCCTTTGCTAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.0	57.8	685-764 bp	1.0	75.0
ndhC-trnV-UAC_p4	TTCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	47.6	50.0	58.0	57.8	684-763 bp	1.0	74.7
ndhC-trnV-UAC_p5	CGAAAATGGGAATAACGCTCG	AGAAGGTCTACGGTTCGAGT	47.6	50.0	58.0	57.8	635-714 bp	1.0	74.5