Hotspot

trnT-UGU-trnL-UAA

LSC · 49636-50292 · 657 bp

Back to Aphanocalyx

Pi 0.0122

MarkerSeek score 65.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 8
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	715-799 bp	1.0	79.2
trnT-UGU-trnL-UAA_p2	TCCAAATAGGGGAAAAGCCG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.9	58.1	773-857 bp	1.0	79.1
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	747-831 bp	1.0	78.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	721-805 bp	1.0	77.9
trnT-UGU-trnL-UAA_p5	TCCAAATAGGGGAAAAGCCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.9	58.2	779-863 bp	1.0	77.8