Hotspot

psaA-ycf3

LSC · 45170-45899 · 730 bp

Back to Aphanocalyx

Pi 0.00822

MarkerSeek score 63.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 6
pi: 0.00822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACAGGGC	50.0	50.0	57.9	58.1	841 bp	1.0	78.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	850 bp	1.0	78.8
psaA-ycf3_p3	GAAAAATGACCGGGTTTGGC	TTGGTTGAAGATCACAGGGC	50.0	50.0	58.2	58.1	859 bp	1.0	78.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACAGGGCGTTTC	50.0	50.0	57.9	58.2	836 bp	1.0	78.1
psaA-ycf3_p5	GAAAAATGACCGGGTTTGGC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.2	58.1	868 bp	1.0	78.0