Hotspot

trnR-UCU-atpA

LSC · 10229-10649 · 421 bp

Back to Aphanocalyx

Pi 0.0214

MarkerSeek score 59.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 9
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	535-556 bp	1.0	66.9
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	539-560 bp	1.0	66.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	525-546 bp	1.0	64.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTTACCGGGGAAGCAGAAG	47.6	50.0	57.3	57.6	534-555 bp	1.0	64.6
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTTACCGGGGAAGCAGAA	47.6	50.0	56.9	57.9	570-591 bp	1.0	64.0